4th November 2019

umass
13

What is the purpose of TLC?

Thin layer chromatography, or TLC, is a method for analyzing mixtures by separating the compounds in the mixture. TLC can be used to help determine the number of components in a mixture, the identity of compounds, and the purity of a compound.

Accordingly, what does the abbreviation TLC stand for in chemistry?

TLC is an abbreviation for Thin Layer Chromatography, and like all types of chromatography, it is an analytical technique used to separate a chemical mixture. TLC is perhaps the most basic of the chromatographic techniques, but is by no means any less useful. Its simplicity is its greatest assest.

What does the RF value tell you in TLC?

The larger an Rf of a compound, the larger the distance it travels on the TLC plate. When comparing two different compounds run under identical chromatography conditions, the compound with the larger Rf is less polar because it interacts less strongly with the polar adsorbent on the TLC plate.

What is the principle of thin layer chromatography?

Chromatography works on the principle that different compounds will have different solubilities and adsorption to the two phases between which they are to be partitioned. Thin Layer Chromatography (TLC) is a solid-liquid technique in which the two phases are a solid (stationary phase) and a liquid (moving phase).